Difference between revisions of "Part:BBa K3286008"
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===Usage and Biology===
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==Fluorescence Loss Assays==   
 
==Fluorescence Loss Assays==   
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<p>characterised by iGEM19_Wageningen</p>
 
  
 
=== dCas9 - monoplex===
 
=== dCas9 - monoplex===

Revision as of 21:02, 21 October 2019


IPTG inducible dCas9 expression module

The expression dCas9 is under control of the constitutive TET promoter. Expression regulation is achieved by the lac operator located between dCas9 and pTET. In the un-induced state, the lacI protein is bound to the lac operator inhibiting expression of the dCas9. Upon addition of the inducer IPTG (or Lactose) the repressor lacI binds to the inducer and dCas9 is being expressed.

pTET: Part:BBa_K3286005

lac operator: Part:BBa_K3286004

dCas9: Part:BBa_K3286012

bi-directional terminator: Part:BBa_K3286006

lacI promoter: Part:BBa_K2572009

lacI: Part:BBa_K143033

Prarthana terminator: Part:BBa_K3286007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]