Difference between revisions of "Part:BBa K3208002"
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<partinfo>BBa_K3208002 short</partinfo> | <partinfo>BBa_K3208002 short</partinfo> | ||
− | This part is an expression module that produces | + | This part is an expression module that produces Carcharin_2. Carcharin_2 is a putative antimicrobial peptide found in the genome of the great white shark. |
Charcharin_1: SRSSRAGLQFPVGRVHRLQRK | Charcharin_1: SRSSRAGLQFPVGRVHRLQRK | ||
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- start codon ATG (74-76) | - start codon ATG (74-76) | ||
− | - | + | - Charcharin_2's CDS (77-139) |
- stop codon (140-142) | - stop codon (140-142) | ||
- T7 terminator (150-197). | - T7 terminator (150-197). | ||
+ | |||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 21:00, 21 October 2019
Expression module that produces Carcharin_2
This part is an expression module that produces Carcharin_2. Carcharin_2 is a putative antimicrobial peptide found in the genome of the great white shark.
Charcharin_1: SRSSRAGLQFPVGRVHRLQRK
The expression module contains:
- T7 promoter (2-20)
- strong RBS (44-66)
- start codon ATG (74-76)
- Charcharin_2's CDS (77-139)
- stop codon (140-142)
- T7 terminator (150-197).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 82
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 106
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 21