Difference between revisions of "Part:BBa K3110011"
 
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This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS.
 
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS.
 
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
 
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
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Latest revision as of 20:59, 21 October 2019


Weak Promoter Medium RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a medium RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 640


Characterization

Due to shortage of time we could not characterize this part.