Difference between revisions of "Part:BBa K2972008"

Revision as of 20:56, 21 October 2019

Quorum sensing(QS) sensitivity test

Introduction

This part is used to test the sensitivity of QS system. To discover the relationship between the components produced by the QS system and the density of cells in the process of cell growth.

When considering the change of signal factor concentration in a single cell, we refer to the model of Maike in 2010. Because our model is in a closed container, we delete the diffusion term from the original model, and improve the solvability. We modified and established our own model according to the actual situation.

Result

In this result, we can clearly see that AHL showed very low growth rate in the earlystage. At a certain time, when the population density reached a certain threshold,AHL showed a sharp increase trend, indicating that quorum sensing has occurred. The growth rate of LuxI also began to increase suddenly, and a large number of products were produced. This result shows that our model is very consistent with the phenomenon of quorum sensing, and can be used as a mathematical description of the cell growth state in the experiment. This model can be great significance to the experimental guidance.

Sequence and Features