Difference between revisions of "Part:BBa K3208001"
| Line 10: | Line 10: | ||
The expression module contains: | The expression module contains: | ||
| − | -T7 promoter | + | |
| − | - strong RBS | + | - T7 promoter (2-20) |
| − | - start codon ATG | + | |
| − | - Charcharin_1's CDS | + | - strong RBS (44-66) |
| − | - stop codon | + | |
| − | - T7 terminator. | + | - start codon ATG (74-76) |
| + | |||
| + | - Charcharin_1's CDS (77-139) | ||
| + | |||
| + | - stop codon (140-142) | ||
| + | |||
| + | - T7 terminator (150-197). | ||
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Revision as of 20:55, 21 October 2019
Expression module that produces Carcharin_1
This part is an expression module that produces Carcharin_1. Carcharin_1 is a putative antimicrobial peptide found in the genome of the great white shark.
Charcharin_1: SRSSRAGLQFPVGRVHRLQRK
The expression module contains:
- T7 promoter (2-20)
- strong RBS (44-66)
- start codon ATG (74-76)
- Charcharin_1's CDS (77-139)
- stop codon (140-142)
- T7 terminator (150-197).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 21