Difference between revisions of "Part:BBa K3112042"

Revision as of 20:54, 21 October 2019

Chromosomal stabilization element group example 1

Our chromosome stabilizing elements consist of two parts: centromeres and tag for screening.

In this example, we try to directly replace the centromere of Saccharomyces cerevisiae.

Before chromosome transferring, we hoped the element group will not have a great impact on the growth and division of cells. It can stabilize this chromosome with stable elements when the other organism of the fusion system is dominant.

In order to meet the relevant iGEM standards for part, we deleted the homologous arms of Saccharomyces cerevisiae on both sides of the part. That doesn't mean we did the same in the experiment. The significance of creating this example is that we hope that later teams can add the stable element of chromosome that can affect cell growth to any position through PCR or other means.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 906
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 797
Illegal BglII site found at 857
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 In this example, we try to directly replace the centromere of Saccharomyces cerevisiae.
-Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.
+Before chromosome transferring, we hoped the element group will not have a great impact on the growth and division of cells. It can stabilize this chromosome with stable elements when the other organism of the fusion system is dominant.
 In order to meet the relevant iGEM standards for part, we deleted the homologous arms of Saccharomyces cerevisiae on both sides of the part. That doesn't mean we did the same in the experiment. The significance of creating this example is that we hope that later teams can add the stable element of chromosome that can affect cell growth to any position through PCR or other means.