Difference between revisions of "Part:BBa K3112041"

 
Line 7: Line 7:
 
This is our second example of testing chromosome stabilizing elements.The insertion site of this element is about 2K away from the centromere of Saccharomyces cerevisiae.This is also an another attempt to insert new centromere directly into the peripheral heterochromatin region. The aim of this attempt is to ensure that the separation site of this chromosome in the fusion system is consistent with that before fusion without affecting the original cell if possible.
 
This is our second example of testing chromosome stabilizing elements.The insertion site of this element is about 2K away from the centromere of Saccharomyces cerevisiae.This is also an another attempt to insert new centromere directly into the peripheral heterochromatin region. The aim of this attempt is to ensure that the separation site of this chromosome in the fusion system is consistent with that before fusion without affecting the original cell if possible.
  
−
Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.
+
Before chromosome transferring, we hoped the element group will not have a great impact on the growth and division of cells. It can stabilize this chromosome with stable elements when the other organism of the fusion system is dominant.
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:53, 21 October 2019


Chromosomal stabilization element group example 2

Our chromosome stabilizing elements consist of two parts: centromeres and tag for screening.

This is our second example of testing chromosome stabilizing elements.The insertion site of this element is about 2K away from the centromere of Saccharomyces cerevisiae.This is also an another attempt to insert new centromere directly into the peripheral heterochromatin region. The aim of this attempt is to ensure that the separation site of this chromosome in the fusion system is consistent with that before fusion without affecting the original cell if possible.

Before chromosome transferring, we hoped the element group will not have a great impact on the growth and division of cells. It can stabilize this chromosome with stable elements when the other organism of the fusion system is dominant. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1346
    Illegal PstI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
    Illegal BglII site found at 1237
    Illegal BglII site found at 1297
    Illegal BglII site found at 1514
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]