Difference between revisions of "Part:BBa K3257003"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3257003 short</partinfo> | <partinfo>BBa_K3257003 short</partinfo> | ||
This part is lacIq promoter and it can be used to initiate the expression of lacI protein. In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold. | This part is lacIq promoter and it can be used to initiate the expression of lacI protein. In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold. | ||
| + | |||
| + | '''Biology''' | ||
| + | [[File:EGFP with lacIq.tiff|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with lacIq promoter measured by 96-well plate reader''' EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 20:50, 21 October 2019
lacIq Promoter
This part is lacIq promoter and it can be used to initiate the expression of lacI protein. In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
Biology
Figure 1. The relative fluorescence intensity of EGFP with lacIq promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]