Difference between revisions of "Part:BBa K3156888"
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<h3 id="CBD">Design</h3>
 
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<p>We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription then </p>
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<p>We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce  </p>
  
 
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<b>Reference</b>
 
<b>Reference</b>
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[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272
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<p>[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272</p>
 
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Revision as of 20:46, 21 October 2019


pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta

Design

We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce

Figure 1.Circuit design of BBa_K3156888(sfgfp reverse).

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Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).

Usage and Biology

Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).

Reference

[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]