Difference between revisions of "Part:BBa K3117004"
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[[File:G4S_4_Linker.gif|thumb|alt text]] | [[File:G4S_4_Linker.gif|thumb|alt text]] | ||
| − | + | This linker consists of four repeats of four times glycin and one time serine. (Gly4Ser)4 linker is one of the most widely used flexible linker that can sustain the bioactivity of fusion proteins. It was used by the team FAU_Erlangen, 2019, to create a bispecific antibody targeting GPA33 on colorectal cancer cells and CD3 on T cells (<partinfo>BBa_K3117029</partinfo>, <partinfo>BBa_K3117026</partinfo>, <partinfo>BBa_K3117030</partinfo>). | |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:46, 21 October 2019
((Glycine)x4-Serine)x4 Linker
This linker consists of four repeats of four times glycin and one time serine. (Gly4Ser)4 linker is one of the most widely used flexible linker that can sustain the bioactivity of fusion proteins. It was used by the team FAU_Erlangen, 2019, to create a bispecific antibody targeting GPA33 on colorectal cancer cells and CD3 on T cells (BBa_K3117029, BBa_K3117026, BBa_K3117030).
Usage and Biology
Our flexible linker is composed of the small and non-polar amino acid glycin (G) and the polar amino acid serine (S). By adjusting the copy number, in this case 4 repeats of GGGGS, the length of this GS linker can be optimized to achieve appropriate separation of the proteins of interest [1]. 4 repeats ensure a high level of flexibility. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]