Difference between revisions of "Part:BBa K3257001"
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Bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator. | Bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator. | ||
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[[File:EGFP with T5.jpeg|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with T5 promoter measured by 96-well plate reader''' EGFP with T5 promoter represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | [[File:EGFP with T5.jpeg|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with T5 promoter measured by 96-well plate reader''' EGFP with T5 promoter represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | ||
Revision as of 20:45, 21 October 2019
T5 Promoter
Bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator.
Biology
Figure 1. The relative fluorescence intensity of EGFP with T5 promoter measured by 96-well plate reader EGFP with T5 promoter represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]