Difference between revisions of "Part:BBa K3110049:Design"
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+ | <li>parts.igem.org/Part:BBa_K1659002 | ||
+ | <li>Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174 | ||
+ | </ul> |
Latest revision as of 20:35, 21 October 2019
YebF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have taken the sequence which iGEM team of Oxford-2015 used (Part:BBa_K1659002) and optimized it using Twist Bioscience codon optimized tool to make it synthesize compatible.
Source
We ordered the sequence from Twist Bioscience along with IL12-FLAG
References
- parts.igem.org/Part:BBa_K1659002
- Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174