Difference between revisions of "Part:BBa K2992021"

Revision as of 20:34, 21 October 2019

Usage

The BgaR-BgaL system of C. perfringens comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent PgaR -PgaL promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the transcriptional terminator found downstream of the stop codon for bgaR PgaR -PgaL group has recently utlised this regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium (Cañadas et al., 2019). In our project, we use the BgaR-BgaL regulatory system (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture. The secondary structure for TbgaR was predicted using the Mfold web server (Zuker, 2003).

Secondary Structure

Characterisation

The Plac system is an inducible promoter system utilizing the lactose operon from C. Perfringens. This allowed us to test the maximum and minimum reporter expression range. See our results page for more information.

References

Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.

Measurement

• [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]