Difference between revisions of "Part:BBa K3156888"
Marshalliu (Talk | contribs) |
Marshalliu (Talk | contribs) |
||
| Line 23: | Line 23: | ||
</html> | </html> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <html> | ||
| + | <figure> | ||
| + | <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/> | ||
| + | <figcaption></p> | ||
| + | <p style="text-align:center;"><b>Figure 1.Repairing result of BBa_K3156888(<i>sfgfp</i> reverse).</b> </p> | ||
| + | |||
| + | </figcaption> | ||
| + | </figure> | ||
<b>Reference</b> | <b>Reference</b> | ||
[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272 | [1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272 | ||
| + | </html> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 20:33, 21 October 2019
pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta
Design
We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription then
Figure 1.Circuit design of BBa_K3156888(sfgfp reverse).
111111111111111111111111111111111111111111111111111111111111111111111111111
Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).
Usage and Biology
Figure 1.Repairing result of BBa_K3156888(sfgfp reverse).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 516 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]