Difference between revisions of "Part:BBa K2971000"

Revision as of 20:33, 21 October 2019

crtE from Deinococcus radiodurans

This part is the gene crtE (dr1395) from the extremophile Deinococcus radiodurans and encodes geranylgeranyl diphosphate synthase (GGPPS). GGPPS catalyze the condensation of farnesyl pyrophosphate (FPP) and isopentenyl pyrophosphate (IPP) into geranylgeranyl diphosphate (GGPP)[1]. GGPP is a precursor for the carotenoid biosynthetic pathway. The sequence has been codon optimized for expression in Escherichia coli and iGEM registry compatibility.

The UiOslo 2019 team used this gene in a composite part (BBa_K2971004) to produce the red pigment lycopene, in Escherichia coli, by inserting it into a vector along with the genes crtB and crtI.

We expressed the protein under the control of arabionose inducible promotor araC. We detected a faint protein band during our SDS analysis; it is likely the expression was not well-optimized.

References 1. Liu, C., Sun, Z., Shen, S., Lin, L., Li, T., Tian, B., & Hua, Y. (2014). Identification and characterization of the geranylgeranyl diphosphate synthase in Deinococcus radiodurans. Letters in Applied Microbiology, 58(3), 219-224. doi:10.1111/lam.12181

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 841
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 43

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2971000 short</partinfo>
-This part is the gene crtE (dr1395) from the extremophile Deinococcus radiodurans and encodes
+This part is the gene <i>crtE</i> (dr1395) from the extremophile <i>Deinococcus radiodurans</i> and encodes
 geranylgeranyl diphosphate synthase (GGPPS). GGPPS catalyze the condensation of farnesyl
 pyrophosphate (FPP) and isopentenyl pyrophosphate (IPP) into geranylgeranyl diphosphate (GGPP)[1].
 GGPP is a precursor for the carotenoid biosynthetic pathway. The sequence has been codon optimized
-for expression in Escherichia coli and iGEM registry compatibility.
+for expression in <i>Escherichia coli</i> and iGEM registry compatibility.
 The UiOslo 2019 team used this gene in a composite part (BBa_K2971004) to produce the red pigment lycopene, in
-Escherichia coli, by expressing it in a vector along with the genes crtB and crtI.
+<i>Escherichia coli</i>, by inserting it into a vector along with the genes <i>crtB</i> and <i>crtI</i>.
-We expressed the protein under the control of arabionose inducible promotor araC. We detected a small induced band  Likely the expression was not high enough.
+We expressed the protein under the control of arabionose inducible promotor araC. We detected a faint protein band during our SDS analysis; it is likely the expression was not well-optimized.