Difference between revisions of "Part:BBa K3046002:Design"
(→References) |
|||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites. | This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites. | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 20:23, 21 October 2019
PLEAPsonB_1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 602
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 343
Illegal BglII site found at 654 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 602
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 602
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
Source
This part is created on the basis of the upstream region of the sonB ORF (Systematic ORF name: An04g05630) of many aspergillus genomes.It has been created using the proHMMoter software, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
References
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.