Difference between revisions of "Part:BBa K3030015"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3030015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3030015 SequenceAndFeatures</partinfo>
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<p></p>
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<h3>Reference</h3>
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Wieland, M., Hartig, J.S. (2008). ‘Improved aptazyme design and in vivo screening enable riboswitching in bacteria.’ Angewandte Chemie, 47(14), pp2604-2607. [Online]. Available from: https://www.ncbi.nlm.nih.gov/pubmed/18270990
  
  

Latest revision as of 20:16, 21 October 2019


theophylline riboswitch with optimized RBS

This is a theophylline-responsive riboswitch containing optimized RBS. The sequence is initially designed by Wieland, M. and Hartig, J. S. (2008) with Shines-Dalgarno (SD) sequence. We optimized the RBS by replacing SD sequence with calculated RBS by thermodynamic modeling. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Wieland, M., Hartig, J.S. (2008). ‘Improved aptazyme design and in vivo screening enable riboswitching in bacteria.’ Angewandte Chemie, 47(14), pp2604-2607. [Online]. Available from: https://www.ncbi.nlm.nih.gov/pubmed/18270990