Difference between revisions of "Part:BBa K3174008:Design"
(→‎Conception and Design)
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The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.  
 
The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.  
 
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Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part and create a registry page to document it.
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Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Strain_Study">procedure</a></html> for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part.
  
 
===Design Notes===
 
===Design Notes===

Revision as of 20:13, 21 October 2019


SYFP2 coding sequence with IS hotspot removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Conception and Design

The Austin_UTexas 2015 iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work here). Kelsey Hu then performed the initial cloning and characterization of the redesigned sequence.
Later the Austin_UTexas 2019 iGEM team retrieved the sequence and Anna Bardenhagen performed propagation experiments replicating Austin_UTexas 2015's procedure for both the original and redesigned SYFP2 sequences. This data was then used to fully characterize the stability of the new part as it compared to the original. A registry page was created to document the sequence and characterization of the new part.

Design Notes

The redesigned sequence must be less prone to IS10 insertion element mutations while still mainting the same amino acid sequence.


Source

The original SYFP2 coding sequence (BBa_K864100)

References