Difference between revisions of "Part:BBa K2908681"
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<center>https://2019.igem.org/wiki/images/a/a5/T--CSU_CHINA--2908681.jpg</center><br/> | <center>https://2019.igem.org/wiki/images/a/a5/T--CSU_CHINA--2908681.jpg</center><br/> | ||
<center>Physical map of s(ESR1)p-miR101spe-EGFP</center><br/> | <center>Physical map of s(ESR1)p-miR101spe-EGFP</center><br/> | ||
| + | The construction method is the same as module1 (BBa_2908671), only except the paired sequence of each inserted fragment, which shows in the following picture.<br/> | ||
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Latest revision as of 20:11, 21 October 2019
s(ESR1)p-miR101spe-EGFP
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
The construction method is the same as module1 (BBa_2908671), only except the paired sequence of each inserted fragment, which shows in the following picture.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 155
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 200
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]