Difference between revisions of "Part:BBa K3257112"

Latest revision as of 20:03, 21 October 2019

T7 Promoter-lox5171-R-PPT-RBS-ChlR_T33X-PBS-U5-R-loxP-T7 Terminator

This part includes the target gene, which is ChlR_T33X (https://parts.igem.org/Part:BBa_K3257035) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1107
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 945
Illegal BsaI.rc site found at 966

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K3257112 short</partinfo>
-This part includes the gene of interest, which is ChlR_T33X (https://parts.igem.org/Part:BBa_K3257035) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.
+This part includes the target gene, which is ChlR_T33X (https://parts.igem.org/Part:BBa_K3257035) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.
 <!-- Add more about the biology of this part here