Difference between revisions of "Part:BBa K3192012:Design"

 
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===Design Notes===
 
===Design Notes===
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<p> This promoter is intended for inducible expression of a coding region. If your desired gene(s) of expression need to be expressed constitutively do not use this promoter. </p>
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<p>This promoter is ideal for high expression at specified intervals, as the addition of IPTG results in high levels of transcription. If metabolic load on your chassis is a concern for you team, this promoter is ideal for controlling the energy used on BrioBrick expression in a chassis. </p>
  
 
===Source===
 
===Source===

Revision as of 20:00, 21 October 2019


T7A1 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is intended for inducible expression of a coding region. If your desired gene(s) of expression need to be expressed constitutively do not use this promoter.

This promoter is ideal for high expression at specified intervals, as the addition of IPTG results in high levels of transcription. If metabolic load on your chassis is a concern for you team, this promoter is ideal for controlling the energy used on BrioBrick expression in a chassis.

Source

Escherichia coli

https://www.pnas.org/content/102/13/4706

References