Difference between revisions of "Part:BBa K3098015"
Hjy199999666 (Talk | contribs) |
Hjy199999666 (Talk | contribs) (→Usage and Biology) |
||
| Line 9: | Line 9: | ||
Add more about the biology of this part here | Add more about the biology of this part here | ||
| + | <!-- --> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:53, 21 October 2019
sfGFP+Avi-tag+His-tag
iGEM19_WHU-China added Avi-tag to C-terminal of sfGFP to confirm the function of BirA enzyme(BBa_K2485001).sfGFP is codon optimized from BBa_I746916.Avi-tag can perform normal function whether added to C-terminal or N-terminal of the peptide.Avi-tag can be specifically recognized by BirA enzyme in vitro/vivo to attach a biotin. Furthermore, this part can be used to produce biotinylated sfGFP for its purification and other signal detection/amplification possibilities in future versatile platform.
Add more about the biology of this part here
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 148 - 1000COMPATIBLE WITH RFC[1000]