Difference between revisions of "Part:BBa K3051421"
AleColosio (Talk | contribs) |
AleColosio (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3051421 short</partinfo> | <partinfo>BBa_K3051421 short</partinfo> | ||
| − | This is a lipase found in a metagenomics search of a compost done by the University of Gottingen. It was found to break down the ester bond of p nitrophenol octanoate. It has a similarity (77% identity) to Thermostable Lipase A (BBa_K258006), and we believe it has the same function. Its conserved domains include a hydrolase site and an RTX toxin related Ca2+ binding site, which may make it sensitive to Ca ions or act as a cofactor. | + | <div style="width:50%;float:left">This is a lipase found in a metagenomics search of a compost done by the University of Gottingen. It was found to break down the ester bond of p nitrophenol octanoate. It has a similarity (77% identity) to Thermostable Lipase A (BBa_K258006), and we believe it has the same function. Its conserved domains include a hydrolase site and an RTX toxin related Ca2+ binding site, which may make it sensitive to Ca ions or act as a cofactor. |
| − | It has a kDa of 64.6 and an estimated molar extinction coefficient of 75290 M-1cm-1 (calculated using ProtoPram ExPaSy tool). It has the following 3D structure (calculated using Phyre 2) | + | It has a kDa of 64.6 and an estimated molar extinction coefficient of 75290 M-1cm-1 (calculated using ProtoPram ExPaSy tool). It has the following 3D structure (calculated using Phyre 2) </div> |
<div>https://2019.igem.org/wiki/images/a/a1/T--Warwick--CMLP_3D_structure.png</div> | <div>https://2019.igem.org/wiki/images/a/a1/T--Warwick--CMLP_3D_structure.png</div> | ||
Revision as of 19:49, 21 October 2019
Compost Lipase
This is a lipase found in a metagenomics search of a compost done by the University of Gottingen. It was found to break down the ester bond of p nitrophenol octanoate. It has a similarity (77% identity) to Thermostable Lipase A (BBa_K258006), and we believe it has the same function. Its conserved domains include a hydrolase site and an RTX toxin related Ca2+ binding site, which may make it sensitive to Ca ions or act as a cofactor.
It has a kDa of 64.6 and an estimated molar extinction coefficient of 75290 M-1cm-1 (calculated using ProtoPram ExPaSy tool). It has the following 3D structure (calculated using Phyre 2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]