Difference between revisions of "Part:BBa K117004:Experience"
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=='''Abnormality found in the registry'''== | =='''Abnormality found in the registry'''== | ||
This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light. | This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light. | ||
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The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated. | The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated. | ||
| + | [[image:NTU@iGEM_Diagram_of_characterization_pLacI-GFP.JPG|thumb|center|400px| The characterization overlook]] | ||
Shown below is the graph obtained for the experiment conducted at 37°C. | Shown below is the graph obtained for the experiment conducted at 37°C. | ||
[[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]] | [[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]] | ||
Revision as of 13:51, 26 October 2008
Contents
Abnormality found in the registry
This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.
.JPG)
Characterization of pLacI-GFP
The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.
Shown below is the graph obtained for the experiment conducted at 37°C.
Electron microscope pictures
For more information including the protocols and results obtained, please go to:
[http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]
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UNIQ75f2be6dbd19cf2e-partinfo-00000000-QINU UNIQ75f2be6dbd19cf2e-partinfo-00000001-QINU