Difference between revisions of "Part:BBa K3156888"
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| − | <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/4/46/T--SHSBNU_China---888.jpeg" width = " | + | <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/4/46/T--SHSBNU_China---888.jpeg" width = "300" height ="100"/> |
<figcaption> | <figcaption> | ||
| − | <b>Figure 1.Circuit design of BBa_K3156888(<i>sfgfp</i>reverse).</b> | + | <b>Figure 1.Circuit design of BBa_K3156888(<i>sfgfp</i> reverse).</b> |
</figcaption> | </figcaption> | ||
Revision as of 19:48, 21 October 2019
pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta
We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription then
<figure>
<img src="
" width = "300" height ="100"/>
<figcaption>
Figure 1.Circuit design of BBa_K3156888(sfgfp reverse).
</figcaption>
</figure>
Usage and Biology
Reference [1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272 Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 9
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
Illegal XhoI site found at 516 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]