Difference between revisions of "Part:BBa K117004:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ==Abnormality found in the registry== |
| + | This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light. | ||
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| + | [[image:NTU@iGEM_24062008_(pink_gfp).JPG|thumb|center|300px| BBa_J04430 glows pink]] | ||
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| + | ==Characterization of pLacI-GFP== | ||
| + | The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated. | ||
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| + | Shown below is the graph obtained for the experiment conducted at 37°C. | ||
| + | [[image:NTU@iGEM_37norm.jpg|thumb|center|400px| Characterization at 37°C]] | ||
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| + | ===Electron microscope pictures=== | ||
| + | [[image:NTU@iGEM_Fluorscence_LacI-GFP_1mM.JPG|thumb|center|300px| Cells induced with 1mM lactose concentration]] | ||
| + | [[image:NTU@iGEM_Fluorscence_LacI-GFP_10mM.JPG|thumb|center|300px| Cells induced with 10mM lactose concentration]] | ||
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| + | For more information including the protocols and results obtained, please go to: | ||
| + | [http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:33, 26 October 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Abnormality found in the registry
This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.
.JPG)
Characterization of pLacI-GFP
The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.
Shown below is the graph obtained for the experiment conducted at 37°C.
Electron microscope pictures
For more information including the protocols and results obtained, please go to:
[http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]
User Reviews
UNIQe3d4ccaf6a760a56-partinfo-00000000-QINU UNIQe3d4ccaf6a760a56-partinfo-00000001-QINU