Difference between revisions of "Part:BBa K3081110"
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<partinfo>BBa_K3081110 short</partinfo> | <partinfo>BBa_K3081110 short</partinfo> | ||
| − | The 19 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | + | The 19 bp sgRNA targets to the R1+ DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> |
Latest revision as of 19:19, 21 October 2019
a 19bp sequence targeting the R1(top strand) DnaA box on E.coli oriC
The 19 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see BBa_K3081058
Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]
Reference:
[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]