Difference between revisions of "Part:BBa K3081062"
 
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https://2019.igem.org/wiki/images/c/cf/T--Peking--plasmid_copy_number_pL01.png
 
https://2019.igem.org/wiki/images/c/cf/T--Peking--plasmid_copy_number_pL01.png
 
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Reference:
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[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:12, 21 October 2019


pBAD-dCas9-J23119-pL01

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IR- 1 region

of plasmid replication origin pSC101,using in the CRISPRri systemBBa_K3081007 to control the plasmid copy number.

T--Peking--plasmid_copy_number_pL01.png


Reference:

[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961