Difference between revisions of "Part:BBa I715039"
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<partinfo>BBa_I715039 SequenceAndFeatures</partinfo> | <partinfo>BBa_I715039 SequenceAndFeatures</partinfo> | ||
| + | <h1>Measured by BIT-China 2019</h1> | ||
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| + | The function of BBa_I715039 is confirmed by our 2019 team. | ||
| + | We successfully constructed pUC19-mRFP plasmid and pUC19 plasmid expressing plasmid and measured the activity of these parts quantitatively. In this time,we introduced both of mRFP expressing plasmid and control group expressing plasmid into the same strain, difference in mRFP expression was observed dependent on cultivation time. | ||
| + | The culture of the strain with pUC19-mRFP at 37℃ showed about 70 folds higher fluorescence intensity than control group, While the culture of the strain with pUC19-mRFP and control group at 37℃ at Initial stage of fermentation showed almost the same fluorescence intensity. No inducer was added during the entire fermentation process.This result indicates that the lac promoter is leaked without the addition of IPTG induction. | ||
| + | |||
| + | [[File:BIT-China bronze 2.png|thumb|center|400px|<b>Fig.Fluorescent fermentation results of I715039.</b>]]<br><br> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 18:58, 21 October 2019
pLac-RBS-RFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Measured by BIT-China 2019
The function of BBa_I715039 is confirmed by our 2019 team. We successfully constructed pUC19-mRFP plasmid and pUC19 plasmid expressing plasmid and measured the activity of these parts quantitatively. In this time,we introduced both of mRFP expressing plasmid and control group expressing plasmid into the same strain, difference in mRFP expression was observed dependent on cultivation time. The culture of the strain with pUC19-mRFP at 37℃ showed about 70 folds higher fluorescence intensity than control group, While the culture of the strain with pUC19-mRFP and control group at 37℃ at Initial stage of fermentation showed almost the same fluorescence intensity. No inducer was added during the entire fermentation process.This result indicates that the lac promoter is leaked without the addition of IPTG induction.
