Difference between revisions of "Part:BBa K3046033"
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This part is a long sequence of DNA that are homologous to a part of the <i>albA</i> conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032). | This part is a long sequence of DNA that are homologous to a part of the <i>albA</i> conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 18:52, 21 October 2019
IS2 downstream sequence for A. niger genome integration
This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 118
Illegal BglII site found at 1360 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 118
Illegal PstI site found at 1262
Illegal PstI site found at 1863
Illegal NgoMIV site found at 1250 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1751
Illegal SapI site found at 2060