Difference between revisions of "Part:BBa K2971002:Design"
| Line 9: | Line 9: | ||
Not include restriction sites to. | Not include restriction sites to. | ||
| + | The gene was selected from Deinococcus radiodurans due to the organism’s extremophilic properties. | ||
| + | D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell (see our | ||
| + | design page), such as a high resistance towards ionizing radiation and dehydration. Although the | ||
| + | translated protein does not necessarily exhibit the same resistances as the whole organism, it was | ||
| + | selected to highlight our interest in D.radiodurans. | ||
| + | The gene was placed in an expression vector under pBAD induction so that we could overexpress the | ||
| + | protein to test its enzymatic activity. Due to time restraints, and the general difficulty of characterizing | ||
| + | carotenogenic enzymes, this was not achieved. | ||
Revision as of 18:49, 21 October 2019
crtB from Deinococcus radiodurans
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 609
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Not include restriction sites to.
The gene was selected from Deinococcus radiodurans due to the organism’s extremophilic properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell (see our design page), such as a high resistance towards ionizing radiation and dehydration. Although the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans. The gene was placed in an expression vector under pBAD induction so that we could overexpress the protein to test its enzymatic activity. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.
Source
Genomic sequence comes from Deinococcus radiodurans.