This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from <i>Francisella tularensis ssp.</i> novicida, which has been adapted to the MoClo standard.
+
This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from <i>Francisella tularensis ssp.</i> novicida, which has been adapted to the MoClo standard. <a href="http://2018.igem.org/Team:Marburg/Design">(Ungerer & Pakrasi, 2016)</a>
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection iGEM Marburg 2018</a>.
+
These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="https://www.nature.com/articles/srep39681">Design of the Marburg Collection iGEM Marburg 2018</a>.
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Revision as of 18:32, 21 October 2019
pMC_0_4_35_cpf1
Sequence and Features
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 633 Illegal XbaI site found at 1356 Illegal SpeI site found at 3622
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1713 Illegal NheI site found at 3433 Illegal SpeI site found at 3622
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 3494
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 633 Illegal XbaI site found at 1356 Illegal SpeI site found at 3622
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 633 Illegal XbaI site found at 1356 Illegal SpeI site found at 3622
1000
COMPATIBLE WITH RFC[1000]
Description
This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from Francisella tularensis ssp. novicida, which has been adapted to the MoClo standard. (Ungerer & Pakrasi, 2016)
Compability
These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.
