Difference between revisions of "Part:BBa K3110020:Design"
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===Source=== | ===Source=== | ||
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Weak promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT <br> | Weak promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT <br> | ||
lldR+lldD was amplified from the genome | lldR+lldD was amplified from the genome | ||
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===References=== | ===References=== | ||
Latest revision as of 18:30, 21 October 2019
Weak Promoter Medium RBS lldR+lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1989
Illegal AgeI site found at 621 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1426
Design Notes
Source
Weak promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT
lldR+lldD was amplified from the genome
References
Reference
iGEM ETH zurich 2015
iGEM NUS Singapore 2016