Difference between revisions of "Part:BBa K3081051"
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We used different concentration of IPTG to regulate the expression level of LuxI. Grown cultures were induced by different concentration of IPTG in M9 medium for 6hours. And we used flow cytometry to measure the single bacterium’s GFP expression level (Figure.1). The cells’ expression level is positively related to the concentration of IPTG. | We used different concentration of IPTG to regulate the expression level of LuxI. Grown cultures were induced by different concentration of IPTG in M9 medium for 6hours. And we used flow cytometry to measure the single bacterium’s GFP expression level (Figure.1). The cells’ expression level is positively related to the concentration of IPTG. | ||
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<center>https://2019.igem.org/wiki/images/4/4d/T--Peking--QS77.jpg </center> | <center>https://2019.igem.org/wiki/images/4/4d/T--Peking--QS77.jpg </center> | ||
| − | <center> | + | <center>Figure 1.Characterization of the quorum sensing system</center> |
Revision as of 18:30, 21 October 2019
Quorum Sensing CRISPRri System Enables Spatial-level Growth Control and Ultrasensitive Autoregulation of Growth
Based on the well-characterized growth control system, we constructed a quorum sensing CRISPRri system (qs-CRISPRri) to realize smart regulation of bacterial overall states. At first, we use GFP as the output to test our system.
We used different concentration of IPTG to regulate the expression level of LuxI. Grown cultures were induced by different concentration of IPTG in M9 medium for 6hours. And we used flow cytometry to measure the single bacterium’s GFP expression level (Figure.1). The cells’ expression level is positively related to the concentration of IPTG.
