Difference between revisions of "Part:BBa K079050"
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This part is a DNA damage reporter. See experience part for more Experimental details. | This part is a DNA damage reporter. See experience part for more Experimental details. | ||
− | + | The Northwestern 2019 iGEM team provided novel characterization data to part BBa_K079050. The 2008 Bologna Team reported their part unable to be induced by UV light, however, after exposing cultures transformed with part BBa_K079050 to various doses of UV-C (0 J/m^2, 50 J/m^2, and 125 J/m^2) we found that their part was completely inducible by UV light. Additionally, the rate of decrease in Fluorescence/OD is significantly different between each exposure time, providing evidence that this part may actually be able to differentiate between different UV doses, just not in the way it was originally designed for (Graph below). Fluorescence measurements were taken every 3 minutes immediately after exposure in a 96-well plate reader for 3 hours. | |
+ | |||
+ | https://2019.igem.org/wiki/images/d/d0/T--Northwestern--LexA2FODgraph.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 18:24, 21 October 2019
GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator
This part is a DNA damage reporter. See experience part for more Experimental details.
The Northwestern 2019 iGEM team provided novel characterization data to part BBa_K079050. The 2008 Bologna Team reported their part unable to be induced by UV light, however, after exposing cultures transformed with part BBa_K079050 to various doses of UV-C (0 J/m^2, 50 J/m^2, and 125 J/m^2) we found that their part was completely inducible by UV light. Additionally, the rate of decrease in Fluorescence/OD is significantly different between each exposure time, providing evidence that this part may actually be able to differentiate between different UV doses, just not in the way it was originally designed for (Graph below). Fluorescence measurements were taken every 3 minutes immediately after exposure in a 96-well plate reader for 3 hours.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 729