Difference between revisions of "Part:BBa K3269001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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uvrA is upstream of a reporter gene to sense DNA damage
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uvrA is upstream of a reporter gene to sense DNA damage. UvrA is one of the genes involved in the SOS response network, and is therefore regulated by LexA and RecA. We chose to use the uvrA gene as our sensor because it is specifically involved in the repair of UV induced DNA lesions, making its expression specific to the amount of that type of damage
  
  

Latest revision as of 18:19, 21 October 2019


uvrA GFP UV Damage Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 586
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 976
    Illegal BsaI site found at 1154


Design Notes

uvrA is upstream of a reporter gene to sense DNA damage. UvrA is one of the genes involved in the SOS response network, and is therefore regulated by LexA and RecA. We chose to use the uvrA gene as our sensor because it is specifically involved in the repair of UV induced DNA lesions, making its expression specific to the amount of that type of damage


Source

uvrA is from E Coli genome


References