Difference between revisions of "Part:BBa K2940007"
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<partinfo>BBa_K2940007 short</partinfo> | <partinfo>BBa_K2940007 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Dye-degrading peroxidase (DyP) is from Bacillus subtilis. This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was referenced from NCBI (NC_000964.3) without any modification. (NC_000964.3) It was cloned into pSB1C3 using classical cloning and was verified using SDS-PAGE analysis and ABTS assay. | ||
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− | + | ===Sequence and Features=== | |
<partinfo>BBa_K2940007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2940007 SequenceAndFeatures</partinfo> | ||
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Revision as of 18:17, 21 October 2019
Secreted dye-degrading peroxidase(DyP)
Usage and Biology
Dye-degrading peroxidase (DyP) is from Bacillus subtilis. This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was referenced from NCBI (NC_000964.3) without any modification. (NC_000964.3) It was cloned into pSB1C3 using classical cloning and was verified using SDS-PAGE analysis and ABTS assay.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 155
Illegal BsaI site found at 164
Illegal BsaI site found at 1130
Illegal SapI site found at 560
Illegal SapI site found at 918
Illegal SapI.rc site found at 1074