Difference between revisions of "Part:BBa K3110028"
 
Line 18: Line 18:
 
<partinfo>BBa_K3110028 parameters</partinfo>
 
<partinfo>BBa_K3110028 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Characterisation===
 +
The construct was not characterised due to insufficient time.

Latest revision as of 18:14, 21 October 2019


Weak Promoter Strong RBS lldP+lldD

This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a strong RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying the strength of the promoter and RBS controlling their production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2858
    Illegal AgeI site found at 464
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1524
    Illegal AgeI site found at 1536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2295


Characterisation

The construct was not characterised due to insufficient time.