Difference between revisions of "Part:BBa K3046005:Design"
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| − | This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: | + | This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An12g07450) of many <i>aspergillus</i> genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model |
===References=== | ===References=== | ||
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019. | [1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019. | ||
Revision as of 18:14, 21 October 2019
PLEAPmstA_1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 61
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 470
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
Source
This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An12g07450) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
References
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.