Difference between revisions of "Part:BBa K3046006:Design"
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===Source=== | ===Source=== | ||
| − | This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: | + | This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An07g08210) of many <i>aspergillus</i> genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model |
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===References=== | ===References=== | ||
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019. | [1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019. | ||
Revision as of 18:13, 21 October 2019
PLEAPunk_1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
Source
This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An07g08210) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
References
[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.