Difference between revisions of "Part:BBa K2974500:Design"
Monicacho123 (Talk | contribs) |
Monicacho123 (Talk | contribs) (→Source) |
||
| Line 20: | Line 20: | ||
<center><i>Figure 2. LacZ Toehold Construct with BBa_J23100 Promoter designed in SnapGene.</i></center> | <center><i>Figure 2. LacZ Toehold Construct with BBa_J23100 Promoter designed in SnapGene.</i></center> | ||
===Source=== | ===Source=== | ||
| − | |||
| − | |||
===References=== | ===References=== | ||
Latest revision as of 18:06, 21 October 2019
Medium Promoter (BBa_J23106) Toehold LacZ
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold was designed with promoter BBa_J23106 and reporter gene BBa_K2550201. This reporter contributed by 2018 Lambert iGEM codes for the full-length LacZ Beta-galactosidase gene with a wobble introduced to ensure that the gene is restriction fragment cloning compatible. In our model, LacZ is the downstream reporter translated following binding of the trigger sequence to the toehold switch. The entire LacZ construct was designed in SnapGene. Due to the size of the LacZ reporter gene and constraints of restriction sites present in the pSB3C5 plasmid, the team decided to use Gibson Assembly rather than restriction digest in cloning workflow. Gibson primers were designed using SnapGene for the J23106 promoter T7 LacZ Toehold.
