Difference between revisions of "Part:BBa K3174008:Design"
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<partinfo>BBa_K3174008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3174008 SequenceAndFeatures</partinfo> | ||
| + | ==Conception and Design== | ||
| + | |||
| + | The 2015 UT iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work <html><a href="http://2015.igem.org/Team:Austin_UTexas/Project/Plasmid_Study">here</a></html>). | ||
===Design Notes=== | ===Design Notes=== | ||
Revision as of 17:59, 21 October 2019
SYFP2 coding sequence with IS hotspot removed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Conception and Design
The 2015 UT iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work here).
Design Notes
the redesigned sequence must be less prone to insertion element mutations while still mainting the same amino acid sequence.
Source
The original SYFP2 coding sequence (BBa_K864100)