Difference between revisions of "Part:BBa K2974500:Experience"
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<strong>Medium Promoter (BBa_J23106) Toehold GFP</strong>
 
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Sequencing of the transformation of part BBa_K2974500 confirmed the toehold construct’s function when it was aligned with the desired toehold sequence. In addition, the results for the toehold was confirmed through a dual plasmid transformation in BL21 E.coli cells. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the dual plasmid transformation with part BBa_K2974700, a toehold construct with a strong promoter (BBa_J23100). The biobricked toehold's (BBa_K2974500) function has been validated through experimentation and sequencing.  
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In order to apply this improvement to part BBa_K2550000, Lambert iGEM re-built the T7 Toehold LacZ construct in SnapGene with part BBa_J23106 as the new promoter. The team then began the cloning workflow using Gibson Assembly, aiming to compare pigmentation of transformed E. coli containing the individual toehold and trigger, as well as a dual plasmid transformation of both. The improved part is BBa_K2974500 and is currently, as of wiki freeze, in the cloning stage, but the team expects to update the registry with the final results after the 2019 Giant Jamboree.
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<strong>Sequence Analysis</strong>
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===Applications of BBa_K2974500===
 
===Applications of BBa_K2974500===

Revision as of 17:58, 21 October 2019


Medium Promoter (BBa_J23106) Toehold GFP
In order to apply this improvement to part BBa_K2550000, Lambert iGEM re-built the T7 Toehold LacZ construct in SnapGene with part BBa_J23106 as the new promoter. The team then began the cloning workflow using Gibson Assembly, aiming to compare pigmentation of transformed E. coli containing the individual toehold and trigger, as well as a dual plasmid transformation of both. The improved part is BBa_K2974500 and is currently, as of wiki freeze, in the cloning stage, but the team expects to update the registry with the final results after the 2019 Giant Jamboree.

Applications of BBa_K2974500

User Reviews

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