Difference between revisions of "Part:BBa K3081116"

Latest revision as of 17:54, 21 October 2019

a kind of 20-bp sgRNA that is designed to be complementary to OriC, the genome replication origin

The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see BBa_K3081058

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]

Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <br> The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2)  on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
+<center>
+https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
+</center>
+<center>
+Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
+</center>
+Reference:
+[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
 <!-- Add more about the biology of this part here