Difference between revisions of "Part:BBa K3110014:Design"
 
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<partinfo>BBa_K3110014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3110014 SequenceAndFeatures</partinfo>
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===Design Notes===
 
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Strong promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT <br>
 
Strong promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT <br>
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lldR+lldD was amplified from the genome
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lldR + lldD was amplified from DH5alpha genome
  
  

Latest revision as of 17:45, 21 October 2019


Strong Promoter Medium RBS lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1989
    Illegal AgeI site found at 621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1426


Source

Strong promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT
lldR + lldD was amplified from DH5alpha genome


References

iGEM ETH zurich 2015
iGEM NUS Singapore 2016
Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07