Difference between revisions of "Part:BBa K3320003"
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<p>This part were cloned on the pUC19 vector, totaling 3752 bp. </p>
 
<p>This part were cloned on the pUC19 vector, totaling 3752 bp. </p>
 
[[File:BBa_K3320003_Map.png|500px|]]
 
[[File:BBa_K3320003_Map.png|500px|]]
−
Figure 1. BBa_K3320003 plasmidial map.png
+
<p>Figure 1. BBa_K3320003 plasmidial map </p>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3320003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3320003 SequenceAndFeatures</partinfo>

Revision as of 17:41, 21 October 2019


pTet + Ellowitz RBS + GFPMut3 + L3S2P22

This composite part produce GFPMut3 induced by pTet promoter.

Usage and Biology

This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system.

Assembly

This part were cloned on the pUC19 vector, totaling 3752 bp.

BBa K3320003 Map.png

Figure 1. BBa_K3320003 plasmidial map

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]