Difference between revisions of "Part:BBa K2956000"
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This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones.
 
This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones.
  
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[[File:T--NYU_New_York--FNS1.png|780px|thumb|left|Figure 1: Conversion of Naringenin to Apigenin in FNS B, measured by gas chromatography]]
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===Usage and Biology===
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Revision as of 17:40, 21 October 2019


RBS-FNS under J23100 Promoter

This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones.

Figure 1: Conversion of Naringenin to Apigenin in FNS B, measured by gas chromatography

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]