Difference between revisions of "Part:BBa K3183010"

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mClover3 is a green fluorescent protein derivative which has been codon optimized for <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
 
mClover3 is a green fluorescent protein derivative which has been codon optimized for <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3183010 SequenceAndFeatures</partinfo>
  
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<!-- Add more about the biology of this part here>
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====Fluorescence wavelengths====
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<br> Bajar ''et al'' report the following excitation and emission data for mClover3 - <br>
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*'''Excitation max''' - 506nm
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*'''Emission max''' - 518nm
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<br>
  
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<!-- Spectrum-->
 
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[[File:BBa_K3183010-F-spectrum.png|thumb|left|430px|'''Figure 1:'''  mClover3 spectrum]]
 
  
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<!-- Results from erm and ldh-->
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<!-- Uncomment this to enable Functional Parameter display>
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[[File:T--Oxford--Results-ldh.png|thumb|right|430px|'''Figure 2:''' - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. <i> Error bars represent Standard error of the mean. n=3 </i>]]
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===Functional Parameters===
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<br><br><br><br><br><br>
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<partinfo>BBa_K3183000 parameters</partinfo>
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<br><br><br>
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<!-- -->
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<br><br><br>
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<br><br><br>
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<!-- Add more about the biology of this part here -->
  
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====Fluorescence wavelengths====
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[[File:BBa_K3183010-F-spectrum.png|thumb|center|430px|'''Figure 1:'''  mClover3 spectrum]] <br>
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Bajar ''et al'' report the following excitation and emission data for mClover3 - <br>
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*'''Excitation max''' - 506nm
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*'''Emission max''' - 518nm
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<br><br>
  
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<!-- -->
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===Parts characterized by Oxford iGEM 2019===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3183010 SequenceAndFeatures</partinfo>
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[[File:BBa_K3183028_mClover3_log_standardcurve.png|thumb|left|430px|'''Figure 2:'''  mClover3 Log Standard curve]]
  
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<!-- Uncomment this to enable Functional Parameter display
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[[File:T--Oxford--Results-ldh.png|thumb|right|430px|'''Figure 2:''' - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. <i> Error bars represent Standard error of the mean. n=3 </i>]]
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===Functional Parameters===
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<br>
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<partinfo>BBa_K3183000 parameters</partinfo>
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<!-- -->
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<br><br>
  
 
===Characterisation===
 
===Characterisation===
  
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This part was characterised in the composite part <partinfo>BBa_K3183101</partinfo>. Data for this part is shown below.
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This part was characterised in the composite part <partinfo>BBa_K3183028</partinfo>, and <partinfo>BBa_K3183101</partinfo>.
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<html>
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    <figure>
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===Use by Team Oxford 2019===
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        <!--img src="https://static.igem.org/mediawiki/2018/5/58/T--Oxford--promoters.png"alt=width="250" height="190" style="float:center">
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This part was used in the following composite parts: <partinfo>BBa_K3183028</partinfo>, <partinfo>BBa_K3183300</partinfo>, <partinfo>BBa_K3183104</partinfo>, <partinfo>BBa_K3183203</partinfo>, <partinfo>BBa_K3183101</partinfo>, and <partinfo>BBa_K3183104</partinfo>.

Revision as of 17:37, 21 October 2019


mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Fluorescence wavelengths


Bajar et al report the following excitation and emission data for mClover3 -

  • Excitation max - 506nm
  • Emission max - 518nm




Figure 1: mClover3 spectrum



Parts characterized by Oxford iGEM 2019

Figure 2: mClover3 Log Standard curve
Figure 2: - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. Error bars represent Standard error of the mean. n=3




Characterisation

This part was characterised in the composite part BBa_K3183028, and BBa_K3183101.

Use by Team Oxford 2019

This part was used in the following composite parts: BBa_K3183028, BBa_K3183300, BBa_K3183104, BBa_K3183203, BBa_K3183101, and BBa_K3183104.