Difference between revisions of "Part:BBa K3081052"

 
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===Quorum Sensing CRISPRri System Enables Spatial-level Growth Control and Ultrasensitive Autoregulation of Growth===
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<partinfo>BBa_K3081052 short</partinfo>
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Quorum sensing system is widely used in gene circuit designed as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activates the transcription of plux. In our project, we want dCas9 to express when bacteria density is high. And we put the GFP gene under the control of plux, and get this composite part. We used this part to test the quorum sensing system. To avoid the homologous recombination, we changed the terminator of GFP to B1002.
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Based on the well-characterized growth control system, we constructed a quorum sensing CRISPRri system (qs-CRISPRri) to realize smart regulation of bacterial overall states. At first, we use GFP as the output to test our system.  
  
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<center>https://2019.igem.org/wiki/images/1/17/T--Peking--QS66-.png</center>
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===Usage and Biology===
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<center>Figure1</center>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3081052 SequenceAndFeatures</partinfo>
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A time-scale quorum sensing system can be transformed to spatial-level through a donor/receiver system (Figure 1). The donor cells, which merely express and release AHL, would activate the GFP expression of receiver cells through AHL diffusion. This is validated on the agar plate, by dropping the donor cells in the center and receiver cells around them with different distances. We found a progressive decrease in fluorescence as the receiver cells locate farther from central AHL donor (Figure 2).
  
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<center>https://2019.igem.org/wiki/images/e/e0/T--Peking--8.jpg</center>
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===Functional Parameters===
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<partinfo>BBa_K3081052 parameters</partinfo>
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<center>Figure2</center>
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Revision as of 17:27, 21 October 2019

Quorum Sensing CRISPRri System Enables Spatial-level Growth Control and Ultrasensitive Autoregulation of Growth

Based on the well-characterized growth control system, we constructed a quorum sensing CRISPRri system (qs-CRISPRri) to realize smart regulation of bacterial overall states. At first, we use GFP as the output to test our system.

T--Peking--QS66-.png
Figure1

A time-scale quorum sensing system can be transformed to spatial-level through a donor/receiver system (Figure 1). The donor cells, which merely express and release AHL, would activate the GFP expression of receiver cells through AHL diffusion. This is validated on the agar plate, by dropping the donor cells in the center and receiver cells around them with different distances. We found a progressive decrease in fluorescence as the receiver cells locate farther from central AHL donor (Figure 2).

T--Peking--8.jpg
Figure2