Difference between revisions of "Part:BBa K2983006"

Latest revision as of 17:27, 21 October 2019

SfiI cloning sites

This part is a cloning adapter containing 2 SfiI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of E. coli cloning selection markers like LacZalpha (BBa_K2448003) or reporter RFP (BBa_J04450) expression cassettes.

Figure 1. General architecture of the Yarrowia lipolytica Type IIS RFC[10]-compatible Loop assembly platform.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 12
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of ''E. coli'' cloning selection markers like LacZalpha ([[BBa_K2448003|BBa_K2448003]]) or reporter RFP ([[BBa_J04450|BBa_J04450]]) expression cassettes.
+The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of ''E. coli'' cloning selection markers like LacZalpha ([[Part:BBa_K2448003|BBa_K2448003]]) or reporter RFP ([[Part:BBa_J04450|BBa_J04450]]) expression cassettes.
 [[File:T--Evry Paris-Saclay--General architecture of the Yarrowia lipolytica Type IIS RFC.png|900px|thumb|left|Figure 1. General architecture of the ''Yarrowia lipolytica'' Type IIS RFC[10]-compatible Loop assembly platform. ]]